Denaturing Action of Typical Anionic Surfactants on Several Proteins

Effects of Several Nonionic and Anionic Surfactants on Cucumber Protoplasts*

The effects of four nonionic and two anionic surfactants on cucumber protoplasts were examined by light and electron microscopy. Two nonionic surfactants, polyoxyethylene (POE) (n=10) nonylphenylether and POE (n=5) laurylether, caused swelling of protoplasts, followed by their bursting. The bursting gave more destructive effects preferentially on lamellar structures of chloroplasts than the swe...

Effect of Inorganic Cations on Bactericidal Activity of Anionic Surfactants.

Voss, J. G. (Procter & Gamble Co., Cincinnati, Ohio). Effect of inorganic cations on bactericidal activity of anionic surfactants. J. Bacteriol. 86:207-211. 1963.-The bactericidal effectiveness of two alkyl benzene sulfonates and of three other types of anionic surfactants against Staphylococcus aureus is increased in the presence of low concentrations of divalent cations, especially alkaline e...

Use of anionic denaturing detergents to purify insoluble proteins after overexpression

BACKGROUND Many proteins form insoluble protein aggregates, called "inclusion bodies", when overexpressed in E. coli. This is the biggest obstacle in biotechnology. Ever since the reversible denaturation of proteins by chaotropic agents such as urea or guanidinium hydrochloride had been shown, these compounds were predominantly used to dissolve inclusion bodies. Other denaturants exist but have...

Interactions between Cationic Starch and Anionic Surfactants

Biological activity and environmental impact of anionic surfactants.

The newest results concerning the biological activity and environmental fate of anionic surfactants are collected and critically evaluated. The chemical and physicochemical parameters related to the biological activity and the field of application are briefly discussed. Examples on the effect of anionic surfactants on the cell membranes, on the activity of enzymes, on the binding to various pro...